N-ethyl Tadalafil

N-ethyl Tadalafil (Homotadalafil) Research Standard

N-ethyl Tadalafil, also known as Homotadalafil, is a synthetic structural analog of the phosphodiesterase type 5 (PDE5) inhibitor tadalafil. Characterized by the substitution of an ethyl group for the methyl group on the piperazinedione ring, this compound is utilized as a specialized analytical reference standard. In laboratory settings, it serves as a critical reagent for impurity profiling, comparative pharmacological studies, and the development of analytical methods for the detection of PDE5-related compounds in controlled research environments.

Technical Specifications

Mechanism of Action & Research Context

N-ethyl Tadalafil functions as a competitive inhibitor of the phosphodiesterase type 5 (PDE5) enzyme. By structurally occupying the catalytic site typically reserved for cyclic guanosine monophosphate (cGMP), it modulates intracellular signaling pathways.

• PDE5 Inhibition: Like its parent compound, it prevents the hydrolysis of cGMP to 5′-GMP, thereby maintaining elevated intracellular levels of cGMP.

• Signaling Modulation: The accumulation of cGMP leads to the activation of protein kinase G (PKG), promoting downstream smooth muscle relaxation and vasodilation.

• Comparative Pharmacology: The ethyl-group modification is of significant interest in SAR (structure-activity relationship) studies, allowing researchers to evaluate how minor changes in the piperazinedione scaffold affect binding affinity and enzyme selectivity.

Research Applications

N-ethyl Tadalafil is a focused reagent utilized for chemical characterization and analytical validation.

Primary fields of in vitro laboratory investigation include:

• Binding Affinity Assays: Utilizing competitive displacement assays to measure the compound’s interaction with the PDE5 catalytic domain.

• Analytical Reference Standard: Serving as a benchmark in HPLC and mass spectrometry to identify, quantify, and characterize potential impurities or unknown substances in research samples.

• Impurity Profiling: Assisting in the mapping of synthesis by-products and structural derivatives within the PDE5 inhibitor class.

• Structural Analysis: Comparing conformational behavior and binding kinetics against tadalafil and other related analogs.

Storage & Handling Guidelines

To maintain the chemical integrity of the material:

• Long-Term Storage: Store in a cool, dry place.

• Liquid Storage: Keep reconstituted solutions refrigerated (2–8 °C).

• Environmental Control: Protect from direct light to prevent photolytic degradation.

• Vial Integrity: Keep the container tightly sealed after each use to minimize atmospheric exposure.

References

• BenchChem. Preliminary Pharmacological Profiling of N-Ethyl Tadalafil: A Technical Guide.

• BenchChem. N-Ethyl Tadalafil: A Comparative Guide to its Validation and Certification as a Reference Standard.

• CymitQuimica. Product Analysis and CAS Data for 1609405-34-6.

Research Use Disclaimer

Research Use Only Disclaimer: This product is developed and distributed strictly as a Research Use Only (RUO) laboratory reference chemical intended exclusively for non-clinical analytical and scientific investigation. It is not an FDA-approved drug, medication, dietary supplement, or food ingredient, and is strictly prohibited for human or animal consumption. Kimera Chems supplies this high-purity research material solely to qualified institutions and professional investigators for authorized laboratory research.