5-Amino-1MQ

5-Amino-1MQ (Chloride) Research Standard

5-Amino-1MQ (5-Amino-1-methylquinolinium chloride) is a synthetic small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT). Unlike peptides, which are chains of amino acids, this compound is a cell-permeable small molecule that functions as an enzyme-pathway modulator. In laboratory research, it is primarily investigated for its role in regulating metabolic homeostasis, mitochondrial bioenergetics, and NAD+ salvage pathways by selectively inhibiting the methylation of nicotinamide.

Image Alt Text: Chemical structure representation of 5-Amino-1MQ (Chloride salt).

Technical Specifications

Mechanism of Action & Research Context

5-Amino-1MQ is studied for its ability to modulate the cellular metabolic environment through targeted enzyme inhibition.

• NNMT Inhibition: The compound acts as a substrate-targeting, selective inhibitor of NNMT. By inhibiting this enzyme, 5-Amino-1MQ prevents the methylation of nicotinamide into 1-methylnicotinamide (1-MNA).

• NAD+ Pathway Modulation: Because NNMT consumes nicotinamide and methyl groups (SAM), its inhibition is hypothesized to preserve nicotinamide for the NAD+ salvage pathway. This potentially supports intracellular NAD+ availability, which is critical for mitochondrial function and cellular energy regulation.

• Metabolic Signaling: Preclinical research utilizes this compound to investigate the suppression of adipogenesis and the improvement of insulin sensitivity in diet-induced obesity (DIO) models. It is frequently employed to map the relationship between NNMT expression and metabolic dysregulation.

Research Applications

5-Amino-1MQ is a specialized reagent essential for modern metabolic and mitochondrial research.

Primary fields of in vitro and in vivo laboratory investigation include:

• NNMT Enzyme Inhibition Studies: Quantifying the IC50 of the compound in various cell-free and cell-based enzyme assays.

• Metabolic Homeostasis Models: Evaluating the effects of NNMT inhibition on lipid metabolism, lipogenesis, and energy expenditure in adipocyte cell lines (e.g., 3T3-L1).

• NAD+ Salvage Pathway Research: Analyzing the downstream effects of nicotinamide preservation on mitochondrial efficiency and oxidative phosphorylation.

• Comparative Pharmacology: Benchmarking 5-Amino-1MQ against other metabolic modulators to delineate the specific role of NNMT in metabolic disease pathology.

Storage & Handling Guidelines

To maintain the chemical stability and experimental reliability of the compound:

• Storage Environment: Store at -20°C in a dry, cool environment for long-term stability.

• Environmental Control: Protect strictly from light and moisture, as chemical degradation can occur upon prolonged environmental exposure.

• Handling: Allow the container to equilibrate to room temperature before opening to minimize condensation. Utilize standard laboratory safety practices and appropriate PPE.

• Aseptic Technique: Maintain a sterile work environment to ensure the integrity of the compound for cell culture applications.

Research Use Disclaimer

Research Use Only Disclaimer: This product is developed and distributed strictly as a Research Use Only (RUO) laboratory reference chemical intended exclusively for non-clinical analytical and scientific investigation. It is not an FDA-approved drug, medical treatment, dietary supplement, or food ingredient, and is strictly prohibited for human or animal consumption. Kimera Chems supplies this high-purity research material solely to qualified institutions and professional investigators for authorized laboratory research.