AICAR (Acadesine)

Identity AICAR is a purine nucleoside analog and a widely used activator of AMP-activated protein kinase (AMPK). It is cell-permeable and, once inside the cell, is phosphorylated to its monophosphate form (ZMP), which mimics AMP and activates AMPK.

Mechanism AICAR (acadesine) is a cell-permeable nucleoside. Following uptake, it is phosphorylated intracellularly to ZMP (AICAR monophosphate), an AMP mimetic that activates AMPK — the central cellular energy-sensing kinase. This allows AMPK pathway activation to be studied without altering the cell’s actual AMP:ATP ratio, which is the basis of its use as a research tool. Research applications

• AMPK pathway activation and kinase-mechanism studies
• Cellular metabolism and metabolic flux analysis
• Energy-sensing / low-energy-signal mimicry models
• Reference compound in metabolic signaling assays
• Analytical reference standard (AICAR is WADA-prohibited as a metabolic modulator; relevant to anti-doping method development)

Handling and storage −20 °C for long-term storage. Keep dry, amber or opaque, tightly closed. Use clean, dry tools for weighing. See lot COA. Analytical Identity and purity established by HPLC-UV with MS confirmation against a CAS 2627-69-2 reference standard. Lot-specific certificate of analysis supplied with every unit. Research use only. Supplied as a research reference material for in-vitro and analytical laboratory use. Not a drug, food, cosmetic, or dietary supplement. Not for use in humans or animals. No dosing information is provided or implied.