MOTS-C

MOTS-C (Mitochondrial-Derived Peptide) Research Standard

MOTS-C is a synthetic mitochondrial-derived peptide (MDP) encoded within the mitochondrial genome. As a short bioactive peptide, it functions as a signaling molecule that facilitates communication between the mitochondria and the nucleus. In laboratory research, MOTS-C is studied for its role in metabolic homeostasis, including the regulation of insulin sensitivity, glucose metabolism, and cellular stress responses. It serves as a specialized reference standard for investigating mitochondrial signaling loops and the therapeutic potential of MDPs in metabolic pathway mapping.

Technical Specifications

Mechanism of Action & Research Context

MOTS-C is unique in its origin, being encoded directly within the 12S rRNA region of the mitochondrial DNA.

• Pathway Interaction: It modulates metabolic signaling by translocating from the mitochondria to the cell nucleus, where it can influence gene expression.

• Signaling Loops: Researchers utilize MOTS-C to observe how it interacts with cytoplasmic and nuclear signaling pathways, specifically those regulating energy metabolism and inflammatory responses.

• Metabolic Homeostasis: It is widely investigated for its potential to improve insulin sensitivity and mitigate metabolic dysfunction in controlled experimental models.

Research Applications

MOTS-C is a pivotal tool for exploring the crosstalk between mitochondrial function and nuclear gene expression.

Primary fields of in vitro laboratory investigation include:

• Mitochondrial Pathway Mapping: Observing peptide-mediated interactions within mitochondrial signaling loops.

• Nuclear Interaction Studies: Investigating the translocation dynamics of the peptide into the cell nucleus and its subsequent effect on transcriptional output.

• Binding Affinity Assays: Measuring the interaction strength between MOTS-C and specific target proteins involved in metabolic regulation.

• Molecular Stability Tests: Testing the integrity and half-life of the peptide chain under varying thermal and pH environments.

Storage & Handling Guidelines

To maintain the chemical stability and experimental viability of the lyophilized peptide:

• Long-Term Storage: Store lyophilized vials in a freezer.

• Short-Term/Reconstituted Material: Keep reconstituted samples in a refrigerator.

• Environmental Control: Store at room temperature (if lyophilized, short-term) and ensure the material is kept away from direct light.

• Aseptic Technique: Utilize clean laboratory equipment and sterile techniques to prevent cross-contamination during weighing and reconstitution.

• Vial Integrity: Keep the container tightly sealed after each use to prevent atmospheric moisture and air entry.

References

• Lee C, et al. (2015) The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance. Cell Metabolism. 21(3):443–454.

• Kumagai H, et al. (2016) MOTS-c Reduces Appetite and Alters Body Composition and Energy Metabolism in Mice. JCI Insight. 1(10):e88029.

• Hashimoto Y, et al. (2018) Mitochondrial-derived peptide MOTS-c improves insulin sensitivity and mitochondrial respiration in skeletal muscle. Am J Physiol Endocrinol Metab. 314(4):E333–E347.

Research Use Disclaimer

Research Use Only Disclaimer: This product is developed and distributed strictly as a Research Use Only (RUO) laboratory reference chemical intended exclusively for non-clinical analytical and scientific investigation. It is not an FDA-approved drug, medical treatment, dietary supplement, or food ingredient, and is strictly prohibited for human or animal consumption. Kimera Chems supplies this high-purity research material solely to qualified institutions and professional investigators for authorized laboratory research.